An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.
Identifieur interne : 000177 ( Main/Exploration ); précédent : 000176; suivant : 000178An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.
Auteurs : Naoki Shigi [Japon] ; Masaki Horitani [Japon] ; Kenjyo Miyauchi [Japon] ; Tsutomu Suzuki [Japon] ; Misao Kuroki [Japon]Source :
- RNA (New York, N.Y.) [ 1469-9001 ] ; 2020.
Descripteurs français
- KwdFr :
- 4-Thiouridine (analogues et dérivés), 4-Thiouridine (métabolisme), ARN de transfert (génétique), Acide glutamique (génétique), Anticodon (génétique), Codon (génétique), Cyanobactéries (génétique), Escherichia coli (génétique), Fer (métabolisme), Glutamine (génétique), Lysine (génétique), Mycobacterium (génétique), Protéines Escherichia coli (génétique), Soufre (métabolisme), Sulfurtransferases (composition chimique), Sulfurtransferases (génétique).
- MESH :
- analogues et dérivés : 4-Thiouridine.
- composition chimique : Sulfurtransferases.
- génétique : ARN de transfert, Acide glutamique, Anticodon, Codon, Cyanobactéries, Escherichia coli, Glutamine, Lysine, Mycobacterium, Protéines Escherichia coli, Sulfurtransferases.
- métabolisme : 4-Thiouridine, Fer, Soufre.
English descriptors
- KwdEn :
- Anticodon (genetics), Codon (genetics), Cyanobacteria (genetics), Escherichia coli (genetics), Escherichia coli Proteins (genetics), Glutamic Acid (genetics), Glutamine (genetics), Iron (metabolism), Lysine (genetics), Mycobacterium (genetics), RNA, Transfer (genetics), Sulfur (metabolism), Sulfurtransferases (chemistry), Sulfurtransferases (genetics), Thiouridine (analogs & derivatives), Thiouridine (metabolism).
- MESH :
- chemical , analogs & derivatives : Thiouridine.
- chemical , chemistry : Sulfurtransferases.
- chemical , genetics : Anticodon, Codon, Escherichia coli Proteins, Glutamic Acid, Glutamine, Lysine, RNA, Transfer, Sulfurtransferases.
- genetics : Cyanobacteria, Escherichia coli, Mycobacterium.
- chemical , metabolism : Iron, Sulfur, Thiouridine.
Abstract
Transfer RNA (tRNA) is an adaptor molecule indispensable for assigning amino acids to codons on mRNA during protein synthesis. 2-thiouridine (s2U) derivatives in the anticodons (position 34) of tRNAs for glutamate, glutamine, and lysine are post-transcriptional modifications essential for precise and efficient codon recognition in all organisms. s2U34 is introduced either by (i) bacterial MnmA/eukaryote mitochondrial Mtu1 or (ii) eukaryote cytosolic Ncs6/archaeal NcsA, and the latter enzymes possess iron-sulfur (Fe-S) cluster. Here, we report the identification of novel-type MnmA homologs containing three conserved Cys residues, which could support Fe-S cluster binding and catalysis, in a broad range of bacteria, including thermophiles, Cyanobacteria, Mycobacteria, Actinomyces, Clostridium, and Helicobacter Using EPR spectroscopy, we revealed that Thermus thermophilus MnmA (TtMnmA) contains an oxygen-sensitive [4Fe-4S]-type cluster. Efficient in vitro formation of s2U34 in tRNALys and tRNAGln by holo-TtMnmA occurred only under anaerobic conditions. Mutational analysis of TtMnmA suggested that the Fe-S cluster is coordinated by the three conserved Cys residues (Cys105, Cys108, and Cys200), and is essential for its activity. Evolutionary scenarios for the sulfurtransferases, including the Fe-S cluster containing Ncs6/NcsA s2U thiouridylases and several distantly related sulfurtransferases, are proposed.
DOI: 10.1261/rna.072066.119
PubMed: 31801798
PubMed Central: PMC7025502
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.</title><author><name sortKey="Shigi, Naoki" sort="Shigi, Naoki" uniqKey="Shigi N" first="Naoki" last="Shigi">Naoki Shigi</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Horitani, Masaki" sort="Horitani, Masaki" uniqKey="Horitani M" first="Masaki" last="Horitani">Masaki Horitani</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga 840-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga 840-8502</wicri:regionArea><wicri:noRegion>Saga 840-8502</wicri:noRegion></affiliation></author><author><name sortKey="Miyauchi, Kenjyo" sort="Miyauchi, Kenjyo" uniqKey="Miyauchi K" first="Kenjyo" last="Miyauchi">Kenjyo Miyauchi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Tsutomu" sort="Suzuki, Tsutomu" uniqKey="Suzuki T" first="Tsutomu" last="Suzuki">Tsutomu Suzuki</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kuroki, Misao" sort="Kuroki, Misao" uniqKey="Kuroki M" first="Misao" last="Kuroki">Misao Kuroki</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:31801798</idno><idno type="pmid">31801798</idno><idno type="doi">10.1261/rna.072066.119</idno><idno type="pmc">PMC7025502</idno><idno type="wicri:Area/Main/Corpus">000182</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000182</idno><idno type="wicri:Area/Main/Curation">000182</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000182</idno><idno type="wicri:Area/Main/Exploration">000182</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.</title><author><name sortKey="Shigi, Naoki" sort="Shigi, Naoki" uniqKey="Shigi N" first="Naoki" last="Shigi">Naoki Shigi</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Horitani, Masaki" sort="Horitani, Masaki" uniqKey="Horitani M" first="Masaki" last="Horitani">Masaki Horitani</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga 840-8502, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga 840-8502</wicri:regionArea><wicri:noRegion>Saga 840-8502</wicri:noRegion></affiliation></author><author><name sortKey="Miyauchi, Kenjyo" sort="Miyauchi, Kenjyo" uniqKey="Miyauchi K" first="Kenjyo" last="Miyauchi">Kenjyo Miyauchi</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Suzuki, Tsutomu" sort="Suzuki, Tsutomu" uniqKey="Suzuki T" first="Tsutomu" last="Suzuki">Tsutomu Suzuki</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kuroki, Misao" sort="Kuroki, Misao" uniqKey="Kuroki M" first="Misao" last="Kuroki">Misao Kuroki</name><affiliation wicri:level="1"><nlm:affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j">RNA (New York, N.Y.)</title><idno type="eISSN">1469-9001</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anticodon (genetics)</term><term>Codon (genetics)</term><term>Cyanobacteria (genetics)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli Proteins (genetics)</term><term>Glutamic Acid (genetics)</term><term>Glutamine (genetics)</term><term>Iron (metabolism)</term><term>Lysine (genetics)</term><term>Mycobacterium (genetics)</term><term>RNA, Transfer (genetics)</term><term>Sulfur (metabolism)</term><term>Sulfurtransferases (chemistry)</term><term>Sulfurtransferases (genetics)</term><term>Thiouridine (analogs & derivatives)</term><term>Thiouridine (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>4-Thiouridine (analogues et dérivés)</term><term>4-Thiouridine (métabolisme)</term><term>ARN de transfert (génétique)</term><term>Acide glutamique (génétique)</term><term>Anticodon (génétique)</term><term>Codon (génétique)</term><term>Cyanobactéries (génétique)</term><term>Escherichia coli (génétique)</term><term>Fer (métabolisme)</term><term>Glutamine (génétique)</term><term>Lysine (génétique)</term><term>Mycobacterium (génétique)</term><term>Protéines Escherichia coli (génétique)</term><term>Soufre (métabolisme)</term><term>Sulfurtransferases (composition chimique)</term><term>Sulfurtransferases (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Thiouridine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Sulfurtransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Anticodon</term><term>Codon</term><term>Escherichia coli Proteins</term><term>Glutamic Acid</term><term>Glutamine</term><term>Lysine</term><term>RNA, Transfer</term><term>Sulfurtransferases</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>4-Thiouridine</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Sulfurtransferases</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cyanobacteria</term><term>Escherichia coli</term><term>Mycobacterium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN de transfert</term><term>Acide glutamique</term><term>Anticodon</term><term>Codon</term><term>Cyanobactéries</term><term>Escherichia coli</term><term>Glutamine</term><term>Lysine</term><term>Mycobacterium</term><term>Protéines Escherichia coli</term><term>Sulfurtransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Iron</term><term>Sulfur</term><term>Thiouridine</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>4-Thiouridine</term><term>Fer</term><term>Soufre</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transfer RNA (tRNA) is an adaptor molecule indispensable for assigning amino acids to codons on mRNA during protein synthesis. 2-thiouridine (s<sup>2</sup>U) derivatives in the anticodons (position 34) of tRNAs for glutamate, glutamine, and lysine are post-transcriptional modifications essential for precise and efficient codon recognition in all organisms. s<sup>2</sup>U34 is introduced either by (i) bacterial MnmA/eukaryote mitochondrial Mtu1 or (ii) eukaryote cytosolic Ncs6/archaeal NcsA, and the latter enzymes possess iron-sulfur (Fe-S) cluster. Here, we report the identification of novel-type MnmA homologs containing three conserved Cys residues, which could support Fe-S cluster binding and catalysis, in a broad range of bacteria, including thermophiles, <i>Cyanobacteria</i>, <i>Mycobacteria</i>, <i>Actinomyces</i>, <i>Clostridium</i>, and <i>Helicobacter</i> Using EPR spectroscopy, we revealed that <i>Thermus thermophilus</i> MnmA (TtMnmA) contains an oxygen-sensitive [4Fe-4S]-type cluster. Efficient in vitro formation of s<sup>2</sup>U34 in tRNA<sup>Lys</sup> and tRNA<sup>Gln</sup> by holo-TtMnmA occurred only under anaerobic conditions. Mutational analysis of TtMnmA suggested that the Fe-S cluster is coordinated by the three conserved Cys residues (Cys105, Cys108, and Cys200), and is essential for its activity. Evolutionary scenarios for the sulfurtransferases, including the Fe-S cluster containing Ncs6/NcsA s<sup>2</sup>U thiouridylases and several distantly related sulfurtransferases, are proposed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31801798</PMID><DateCompleted><Year>2020</Year><Month>06</Month><Day>19</Day></DateCompleted><DateRevised><Year>2020</Year><Month>06</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-9001</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>03</Month></PubDate></JournalIssue><Title>RNA (New York, N.Y.)</Title><ISOAbbreviation>RNA</ISOAbbreviation></Journal><ArticleTitle>An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.</ArticleTitle><Pagination><MedlinePgn>240-250</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1261/rna.072066.119</ELocationID><Abstract><AbstractText>Transfer RNA (tRNA) is an adaptor molecule indispensable for assigning amino acids to codons on mRNA during protein synthesis. 2-thiouridine (s<sup>2</sup>U) derivatives in the anticodons (position 34) of tRNAs for glutamate, glutamine, and lysine are post-transcriptional modifications essential for precise and efficient codon recognition in all organisms. s<sup>2</sup>U34 is introduced either by (i) bacterial MnmA/eukaryote mitochondrial Mtu1 or (ii) eukaryote cytosolic Ncs6/archaeal NcsA, and the latter enzymes possess iron-sulfur (Fe-S) cluster. Here, we report the identification of novel-type MnmA homologs containing three conserved Cys residues, which could support Fe-S cluster binding and catalysis, in a broad range of bacteria, including thermophiles, <i>Cyanobacteria</i>, <i>Mycobacteria</i>, <i>Actinomyces</i>, <i>Clostridium</i>, and <i>Helicobacter</i> Using EPR spectroscopy, we revealed that <i>Thermus thermophilus</i> MnmA (TtMnmA) contains an oxygen-sensitive [4Fe-4S]-type cluster. Efficient in vitro formation of s<sup>2</sup>U34 in tRNA<sup>Lys</sup> and tRNA<sup>Gln</sup> by holo-TtMnmA occurred only under anaerobic conditions. Mutational analysis of TtMnmA suggested that the Fe-S cluster is coordinated by the three conserved Cys residues (Cys105, Cys108, and Cys200), and is essential for its activity. Evolutionary scenarios for the sulfurtransferases, including the Fe-S cluster containing Ncs6/NcsA s<sup>2</sup>U thiouridylases and several distantly related sulfurtransferases, are proposed.</AbstractText><CopyrightInformation>© 2020 Shigi et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shigi</LastName><ForeName>Naoki</ForeName><Initials>N</Initials><Identifier Source="ORCID">0000-0003-4452-8264</Identifier><AffiliationInfo><Affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Horitani</LastName><ForeName>Masaki</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-8881-0600</Identifier><AffiliationInfo><Affiliation>Faculty of Agriculture, Department of Applied Biochemistry and Food Science, Saga University, 1 Honjo-machi, Saga 840-8502, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Miyauchi</LastName><ForeName>Kenjyo</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Suzuki</LastName><ForeName>Tsutomu</ForeName><Initials>T</Initials><Identifier Source="ORCID">0000-0002-9731-1731</Identifier><AffiliationInfo><Affiliation>Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kuroki</LastName><ForeName>Misao</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-ku, Tokyo 135-0064, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>12</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>RNA</MedlineTA><NlmUniqueID>9509184</NlmUniqueID><ISSNLinking>1355-8382</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C105273">2-thiouridine</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000926">Anticodon</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003062">Codon</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029968">Escherichia coli Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0RH81L854J</RegistryNumber><NameOfSubstance UI="D005973">Glutamine</NameOfSubstance></Chemical><Chemical><RegistryNumber>13957-31-8</RegistryNumber><NameOfSubstance UI="D013891">Thiouridine</NameOfSubstance></Chemical><Chemical><RegistryNumber>3KX376GY7L</RegistryNumber><NameOfSubstance UI="D018698">Glutamic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>70FD1KFU70</RegistryNumber><NameOfSubstance UI="D013455">Sulfur</NameOfSubstance></Chemical><Chemical><RegistryNumber>9014-25-9</RegistryNumber><NameOfSubstance UI="D012343">RNA, Transfer</NameOfSubstance></Chemical><Chemical><RegistryNumber>E1UOL152H7</RegistryNumber><NameOfSubstance UI="D007501">Iron</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.61</RegistryNumber><NameOfSubstance UI="C510958">MnmA protein, E coli</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.8.1.-</RegistryNumber><NameOfSubstance UI="D013466">Sulfurtransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>K3Z4F929H6</RegistryNumber><NameOfSubstance UI="D008239">Lysine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000926" MajorTopicYN="N">Anticodon</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003062" MajorTopicYN="N">Codon</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000458" MajorTopicYN="N">Cyanobacteria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029968" MajorTopicYN="N">Escherichia coli Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018698" MajorTopicYN="N">Glutamic Acid</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005973" MajorTopicYN="N">Glutamine</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007501" MajorTopicYN="N">Iron</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008239" MajorTopicYN="N">Lysine</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009161" MajorTopicYN="N">Mycobacterium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012343" MajorTopicYN="N">RNA, Transfer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013455" MajorTopicYN="N">Sulfur</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013466" MajorTopicYN="N">Sulfurtransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013891" MajorTopicYN="N">Thiouridine</DescriptorName><QualifierName UI="Q000031" MajorTopicYN="N">analogs & derivatives</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">biosynthesis</Keyword><Keyword MajorTopicYN="Y">iron–sulfur cluster</Keyword><Keyword MajorTopicYN="Y">post-transcriptional modification</Keyword><Keyword MajorTopicYN="Y">sulfurtransferase</Keyword><Keyword MajorTopicYN="Y">tRNA</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>12</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pmc-release"><Year>2021</Year><Month>03</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>6</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31801798</ArticleId><ArticleId IdType="pii">rna.072066.119</ArticleId><ArticleId IdType="doi">10.1261/rna.072066.119</ArticleId><ArticleId IdType="pmc">PMC7025502</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proteins. 1994 Dec;20(4):347-55</Citation><ArticleIdList><ArticleId IdType="pubmed">7731953</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA Biol. 2014;11(12):1495-507</Citation><ArticleIdList><ArticleId IdType="pubmed">25607529</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11730-4</Citation><ArticleIdList><ArticleId IdType="pubmed">1334546</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2012 Mar 2;416(4):467-85</Citation><ArticleIdList><ArticleId IdType="pubmed">22227389</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2008 Oct 10;283(41):27469-76</Citation><ArticleIdList><ArticleId IdType="pubmed">18664566</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Inform. 2010;24:104-15</Citation><ArticleIdList><ArticleId IdType="pubmed">22081593</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Inorg Chem. 1999 Dec;4(6):727-41</Citation><ArticleIdList><ArticleId IdType="pubmed">10631604</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell Biol. 2008 May;28(10):3301-12</Citation><ArticleIdList><ArticleId IdType="pubmed">18332122</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2006 Jan 6;21(1):97-108</Citation><ArticleIdList><ArticleId IdType="pubmed">16387657</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1983 Sep 25;258(18):11106-11</Citation><ArticleIdList><ArticleId IdType="pubmed">6309830</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2018 Nov 01;9:2679</Citation><ArticleIdList><ArticleId IdType="pubmed">30450093</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2016 Jul 8;44(W1):W242-5</Citation><ArticleIdList><ArticleId IdType="pubmed">27095192</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1983 Dec 10;258(23):14284-93</Citation><ArticleIdList><ArticleId IdType="pubmed">6315725</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2007 Nov 1;23(21):2947-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17846036</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1985 Oct 8;24(21):5711-5</Citation><ArticleIdList><ArticleId IdType="pubmed">3853464</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 1970 Aug 24;40(4):866-72</Citation><ArticleIdList><ArticleId IdType="pubmed">4924671</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Mar 12;458(7235):228-32</Citation><ArticleIdList><ArticleId IdType="pubmed">19145231</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1974 Jul 1;43(1):59-63</Citation><ArticleIdList><ArticleId IdType="pubmed">4369142</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1996 Jul 5;271(27):16068-74</Citation><ArticleIdList><ArticleId IdType="pubmed">8663056</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Soc Trans. 2018 Dec 17;46(6):1593-1603</Citation><ArticleIdList><ArticleId IdType="pubmed">30381339</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Commun. 2016 Jan 21;7:10457</Citation><ArticleIdList><ArticleId IdType="pubmed">26791911</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2001 Sep 3;20(17):4794-802</Citation><ArticleIdList><ArticleId IdType="pubmed">11532943</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2005 Jan 14;280(2):1613-24</Citation><ArticleIdList><ArticleId IdType="pubmed">15509579</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2016 Aug;73(16):3075-95</Citation><ArticleIdList><ArticleId IdType="pubmed">27094388</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1973 Oct 23;12(22):4331-7</Citation><ArticleIdList><ArticleId IdType="pubmed">4584321</ArticleId></ArticleIdList></Reference><Reference><Citation>Scand J Clin Lab Invest. 2003;63(7-8):489-96</Citation><ArticleIdList><ArticleId IdType="pubmed">14743958</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA. 2007 Aug;13(8):1245-55</Citation><ArticleIdList><ArticleId IdType="pubmed">17592039</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2016 Nov 8;113(45):12703-12708</Citation><ArticleIdList><ArticleId IdType="pubmed">27791189</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteins. 2013 Jul;81(7):1232-44</Citation><ArticleIdList><ArticleId IdType="pubmed">23444054</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1977 Nov 10;252(21):7424-6</Citation><ArticleIdList><ArticleId IdType="pubmed">410804</ArticleId></ArticleIdList></Reference><Reference><Citation>J Bacteriol. 2015 Jun;197(11):1952-62</Citation><ArticleIdList><ArticleId IdType="pubmed">25825430</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Genet. 2014 Apr 02;5:67</Citation><ArticleIdList><ArticleId IdType="pubmed">24765101</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1987 Jun 25;262(18):8488-95</Citation><ArticleIdList><ArticleId IdType="pubmed">3298234</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1994 Jun 28;33(25):7869-76</Citation><ArticleIdList><ArticleId IdType="pubmed">7516708</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5459-64</Citation><ArticleIdList><ArticleId IdType="pubmed">18391219</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2008 Dec 17;27(24):3267-78</Citation><ArticleIdList><ArticleId IdType="pubmed">19037260</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2018 Jan 4;46(D1):D303-D307</Citation><ArticleIdList><ArticleId IdType="pubmed">29106616</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11067-72</Citation><ArticleIdList><ArticleId IdType="pubmed">23776221</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1989;180:51-62</Citation><ArticleIdList><ArticleId IdType="pubmed">2482430</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 2016 Dec;590(24):4628-4637</Citation><ArticleIdList><ArticleId IdType="pubmed">27878988</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2017 Apr 26;139(16):5857-5864</Citation><ArticleIdList><ArticleId IdType="pubmed">28368583</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2004 Sep 28;43(38):12220-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15379560</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA Biol. 2017 Sep 2;14(9):1209-1222</Citation><ArticleIdList><ArticleId IdType="pubmed">28277930</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):7355-7360</Citation><ArticleIdList><ArticleId IdType="pubmed">28655838</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2001 Sep 3;20(17):4863-73</Citation><ArticleIdList><ArticleId IdType="pubmed">11532950</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Inorg Chem. 2000 Jun;5(3):369-80</Citation><ArticleIdList><ArticleId IdType="pubmed">10907748</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 May 19;281(20):14296-306</Citation><ArticleIdList><ArticleId IdType="pubmed">16547008</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 2015;560:19-28</Citation><ArticleIdList><ArticleId IdType="pubmed">26253964</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Rev. 2014 Apr 23;114(8):4229-317</Citation><ArticleIdList><ArticleId IdType="pubmed">24476342</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Jul 27;442(7101):419-24</Citation><ArticleIdList><ArticleId IdType="pubmed">16871210</ArticleId></ArticleIdList></Reference><Reference><Citation>Genes Cells. 2000 Sep;5(9):689-98</Citation><ArticleIdList><ArticleId IdType="pubmed">10971651</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1998 Nov 20;284(1):33-42</Citation><ArticleIdList><ArticleId IdType="pubmed">9811540</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1985 Aug;82(15):4905-9</Citation><ArticleIdList><ArticleId IdType="pubmed">3860833</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303</Citation><ArticleIdList><ArticleId IdType="pubmed">29788355</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA. 1999 Feb;5(2):188-94</Citation><ArticleIdList><ArticleId IdType="pubmed">10024171</ArticleId></ArticleIdList></Reference><Reference><Citation>J Magn Reson. 2006 Jan;178(1):42-55</Citation><ArticleIdList><ArticleId IdType="pubmed">16188474</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell. 2015 Jun 18;161(7):1606-18</Citation><ArticleIdList><ArticleId IdType="pubmed">26052047</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2006 Jan 27;281(4):2104-13</Citation><ArticleIdList><ArticleId IdType="pubmed">16317006</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2013 Oct 23;425(20):3888-906</Citation><ArticleIdList><ArticleId IdType="pubmed">23727144</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2005 Jul 22;19(2):235-46</Citation><ArticleIdList><ArticleId IdType="pubmed">16039592</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2004 Aug 13;279(33):34123-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15181002</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2003 Feb 4;42(4):1109-17</Citation><ArticleIdList><ArticleId IdType="pubmed">12549933</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2014 Jun 06;9(6):e99104</Citation><ArticleIdList><ArticleId IdType="pubmed">24906001</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1985 Apr 22;183(2):206-10</Citation><ArticleIdList><ArticleId IdType="pubmed">2985428</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2012 Oct 26;287(44):36683-92</Citation><ArticleIdList><ArticleId IdType="pubmed">22904325</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2011 Feb 1;27(3):343-50</Citation><ArticleIdList><ArticleId IdType="pubmed">21134891</ArticleId></ArticleIdList></Reference><Reference><Citation>Microorganisms. 2018 Oct 20;6(4):</Citation><ArticleIdList><ArticleId IdType="pubmed">30347855</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2017 May 9;114(19):4954-4959</Citation><ArticleIdList><ArticleId IdType="pubmed">28439027</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1968 Sep 13;161(3846):1146-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17812290</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1975 Oct 10;250(19):7842-7</Citation><ArticleIdList><ArticleId IdType="pubmed">170272</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2009 Mar;37(4):1335-52</Citation><ArticleIdList><ArticleId IdType="pubmed">19151091</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Shigi, Naoki" sort="Shigi, Naoki" uniqKey="Shigi N" first="Naoki" last="Shigi">Naoki Shigi</name></region><name sortKey="Horitani, Masaki" sort="Horitani, Masaki" uniqKey="Horitani M" first="Masaki" last="Horitani">Masaki Horitani</name><name sortKey="Kuroki, Misao" sort="Kuroki, Misao" uniqKey="Kuroki M" first="Misao" last="Kuroki">Misao Kuroki</name><name sortKey="Miyauchi, Kenjyo" sort="Miyauchi, Kenjyo" uniqKey="Miyauchi K" first="Kenjyo" last="Miyauchi">Kenjyo Miyauchi</name><name sortKey="Suzuki, Tsutomu" sort="Suzuki, Tsutomu" uniqKey="Suzuki T" first="Tsutomu" last="Suzuki">Tsutomu Suzuki</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/IronSulferCluV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000177 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000177 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= IronSulferCluV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31801798
|texte= An ancient type of MnmA protein is an iron-sulfur cluster-dependent sulfurtransferase for tRNA anticodons.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31801798" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a IronSulferCluV1
| This area was generated with Dilib version V0.6.38. |  |